TY - JOUR AU - Flórez Pardo, Luz Marina AU - Parra Paz, Angela Sofía AU - López Galán, Jorge Enrique AU - Figueroa Oviedo, Jersson Ivan PY - 2015/12/15 Y2 - 2024/03/29 TI - Using a mediator system to increase the delignification of sugarcane residues with fungal enzymes JF - CT&F - Ciencia, Tecnología y Futuro JA - CT&F Cienc. Tecnol. Futuro VL - 6 IS - 2 SE - Scientific and Technological Research Articles DO - 10.29047/01225383.22 UR - https://ctyf.journal.ecopetrol.com.co/index.php/ctyf/article/view/350 SP - 81-92 AB - <p>Industrial residues are resources that generate regional energy security but they have not been sufficiently valued. In southern Colombia, the sugar cane sector produces approximately 9´000 000 T/year of residues, mostly represented by tops and leaves. Delignification is a critical step in the process of obtaining ethanol from these residues. The removal of lignin using extracellular enzyme extracts and mediators has not been widely studied. Therefore, a methodology was developed to extract, a cocktail of enzymes consisting of laccase, manganese peroxidase (MnP) and lignoperoxidase (LiP) from Pleurotus ostreatus, which was cultivated by solid substrate fermentation. The extracts with two mediators: 1) ABTS: 2,2´-azino-bis-3-ethylbenzthiazoline-6-sulfonic-acid and 2) vanillin, were tested on two different types of residues: untreated residues and pretreated residues with ultrasound. It was found that the crude enzyme extract contained laccase (0.432 U/mL), LiP (0.116 U/mL) and MnP (0.025 U/mL) activity. Additionally, the results confirmed that this extract was capable of removing 52.7% of lignin from ultrasound pretreated sugar cane residues and ABTS as the laccase mediator. It is important to highlight that the results obtained were very promising mainly if a more concentrated extracellular extract can be used.</p> ER -