Isolation of microorganisms with capability to degrade polycyclic aromatic hydrocarbons (PAH'S)

  • N. E. RAMÍREZ Ecopetrol S.A. – Instituto Colombiano del Petróleo, A.A. 4185 Bucaramanga, Santander, Colombia
  • M. C. VARGAS Ecopetrol S.A. – Instituto Colombiano del Petróleo, A.A. 4185 Bucaramanga, Santander, Colombia
  • S. M. RUEDA Ecopetrol S.A. – Instituto Colombiano del Petróleo, A.A. 4185 Bucaramanga, Santander, Colombia
  • F. N. SÁNCHEZ Ecopetrol S.A. – Instituto Colombiano del Petróleo, A.A. 4185 Bucaramanga, Santander, Colombia
Keywords: PAH's, Microcosm, Quemostat, biotratament

Abstract

This paper summarizes a work conducted on the isolation of microorganisms from PAH's contaminated sediments. The methodology involved two selection systems called fast route and slow route in which exposure periods and contaminant concentrations are the key determinants. The microorganisms isolated through the slow route system are more likely to be successful in degrading high molecular weight PAH's. The six strains obtained through the fast route system were able to grow on low molecular weight PAH's showing preference towards the first four EPA (Environmental Protection Agency) priority PAH's.

References

Aamand, J., Bruntse, G., Jepsen, M., Jorgensen, C. and Jensen, B., 1995, "Degradation of PAH's in Soil by Indigenous and Inoculated Bacteria", in Bioaugmentation for Site remediation, Hinchee, R., Fredrickson, J. and Alleman B. (eds.), USA. Battelle Press: 121 - 127.

Biolog, Inc., 1993, "MicroStationTM System Release" 3.50. USA.

Cerniglia, C., 1992. "Biodegradation of Polycyclic aromatic hydrocarborns", Biodegradation, 3: 351 - 368. https://doi.org/10.1007/BF00129093

Cerniglia, C, 1992. "Biodegradation of Polycyclic aromatic hydrocarborns",Biodegradation, 3: 351 - 368. https://doi.org/10.1007/BF00129093

Crawford, R., Oreillu, K. and Tao, H., 1989. "Microorganism Stabilization for in Situ Degradation of Toxic Chemicals", in Biotechnology and Biodegradation, Kamely, D., Chakrabarty, A. and Omenn, G. (eds.), 4: 203 - 209.

Cutright, T. and Lee, S., 1994. "Bioremediation: A competitive Alternative for the cleacup of contaminated MGP sites", Energy Sources, 16 (2): 269 - 277. https://doi.org/10.1080/00908319408909077

Gorden, R., Hazen, T. and Fliermans, C., 1993. "Rapid screening for bacteria capable of degrading toxic organic compounds", Journal of Microbiological Methods, 18: 339 - 347. https://doi.org/10.1016/0167-7012(93)90015-A

Govindaswami, M., Feldhake, D., Kinkle, B., Mindell, D. and Loper, J., 1995. "Phylogenetic Comparison of Two Polycyclic Aromatic Hydrocarbons Degrading Mycobacteria",Applied Environ. Microbiol., 61(9): 3221 - 3226. https://doi.org/10.1128/aem.61.9.3221-3226.1995

Grifoll, M., Selinov, S., Gatlin, C. H. and Chapman, P., 1995. "Actions of a Versatile Fluorence - Degrading Bacterial Isolate on Polycyclic Aromatic Compounds", Applied Environ. Microbiol., 61 (10): 3711 - 3723. https://doi.org/10.1128/aem.61.10.3711-3723.1995

Heitkamp, M. and Cerniglia, C., 1988. "Mineralization of Polycyclic Aromatic Hydrocarbons by a Bacterium Isolated from Sediment below an Oil Field", Applied Environ. Microbiol., 54 (6): 1612 - 1614. https://doi.org/10.1128/aem.54.6.1612-1614.1988

Heitkamp, M., Franklin, W. and Cerniglia, C., 1988. "Microbial Metabolism of Polycyclic Aromatic Hydrocarbons: Isolation and Characterization of a Pyrene -Degrading Bacterium", Applied Environ. Microbiol., 54 (10): 2549 - 2555. https://doi.org/10.1128/aem.54.10.2549-2555.1988

Kiyohara, H., Nagao, K. and Yana, K., 1982. "Rapid screen for bacteria degrading water-insoluble, solid hydrocarbons on agar plates" applied Environ, Microbiol., 42: 454 - 457. https://doi.org/10.1128/aem.43.2.454-457.1982

La Belle, B. E. and Hadley, F. V., 1994 "Biobeware Constrains and considerations when demostrating bioremediation technologies in the field", Journal of Soil Contamination, 3 (2): 119 - 126. https://doi.org/10.1080/15320389409383457

Lisowska, K., Persson, Y., Rumisowska, A. and Parniewski, P., 194. "Degradation of anthracene by a Pseudomonas sp. and Pseudomonas mendocina at low Temperature", Institution of Chemical Engineers Symposium series, publ. by inst. of Chemical Engineer 155-157.

Ramírez, N., Vargas, M.C. and Sánchez. F., 1996. "Use of the Sediemente-Chromotest for monitoring Stimulated Hydrocarbon Biodegradation Processes", Environmental Toxicology and Water Quality, 11: 223 - 230. https://doi.org/10.1002/(SICI)1098-2256(1996)11:3<223::AID-TOX7>3.0.CO;2-B

How to Cite
RAMÍREZ, N. E. ., VARGAS, M. C. ., RUEDA, S. M. ., & SÁNCHEZ, F. N. . (1996). Isolation of microorganisms with capability to degrade polycyclic aromatic hydrocarbons (PAH’S). CT&F - Ciencia, Tecnología Y Futuro, 1(2), 45–53. https://doi.org/10.29047/01225383.597

Downloads

Download data is not yet available.
Published
1996-12-31
Section
Scientific and Technological Research Articles

Altmetric

Crossref Cited-by logo
QR Code