Producción de biosurfactanes (rhamnolipidos) por medio de b pseudomonas aeruginosa aisladas de lodos contaminados en Colombia
Resumen
Se determinó la capacidad de producción de biosurfactantes de cepas de Pseudomonas aeruginosa provenientes de lodos contaminados con hidrocarburo de diferentes regiones del país. La metodología utilizada comprendió el aislamiento de microorganismos, estandarización de condiciones de cultivo por lotes para una buena producción de tensoactivo y caracterización del ramnolípido producido. Se hizo énfasis en la evaluación de las fuentes de carbono más eficientes relacionada con las curvas de crecimiento y producción. También se determinó la estabilidad del ramnolípido frente a las variaciones de pH, temperatura y salinidad. La cepa Pseudomonas aeruginosa BS 3 permite producir biosurfactante del tipo ramnolípido con rendimientos hasta de 2 g-dm-3 con tensión superficial de 30-32 mN-m-1 en cultivos por lotes con nutrientes grado comercial.
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