Biodesulfurization process evaluation with a Gordona rubropertinctus strain

  • Julia Acero Ecopetrol S.A. – Instituto Colombiano del Petróleo, A.A. 4185 Bucaramanga, Santander, Colombia
  • Claudia Berdugo Ecopetrol S.A. – Instituto Colombiano del Petróleo, A.A. 4185 Bucaramanga, Santander, Colombia
  • Leonardo Mogollón Ecopetrol S.A. – Instituto Colombiano del Petróleo, A.A. 4185 Bucaramanga, Santander, Colombia
Keywords: biodesulfurization, non-aqueous biocatalysis, sulfur, SOx

Abstract

Direct combustion of fossil fuels produce sulfur oxides which are the main source of acid rain; therefore, most countries worldwide are regulating its release into the environment. As a consequence, several processes have been developed over the past years for desulfurization of crude oil and distillates. Due to its specificity, biodesulfurization is an interesting alternative for the transformation and upgrading of refined products, acting as a complement to traditional refining processes. This work presents an overview of Ecopetrol - Instituto Colombiano del Petróleo (ICP) efforts to develop a Biodesulfurization process, based on the activity of a native strain of Gordona rubropertinctus ICP172. Technical improvements on the isolation and characterization of desulfurizing microorganisms, the potential of developing new biocatalysts by means of directed evolution techniques, as well as the experience achieved during production of the biocatalyst in large-scale fermentation processes are hereby presented. The results of biodesulfurization reactions in conventional reactors and in a new membrane bioreactor prototype are also included. Finally, technological challenges faced by biodesulfurization processes are also discussed.

References

Acero, J. R. and Mogollón, L., 2002. "Desarrollo de biocatalizadores hidrofóbicos y termotolerantes mediante técnicas de evolución dirigida". Revista Colombiana de Biotecnología, Vol IV (1): 14-20.

Acero, J., 2002. "Development of DBT-monooxygenase mutants by directed evolution for organic sulfur removal". Proceedings 9th Int Petroleum Environmental Conference, October, Albuquerque, NM, USA.

Arnold, F. H., Wintrode, P. C., Miyazaki, K. and Gershenson, A., 2001. "How enzymes adapt: lessons from directed evoluction". Trends in Bioch. Sci., 26: 100-106. https://doi.org/10.1016/S0968-0004(00)01755-2

Berdugo, C., Caballero, C. and Godoy, R. D., 2002. "Aqueous-organic phases separation by membrane reactors in biodesulfurization reactions". CTYF-Ciencia, Tecnología and Futuro, 2 (3): 97-112.

Berdugo, C., Mena, J., Acero, J. and Mogollón, L., 2001. "Increasing the production of desulfurizing biocatalysts by means of fed-batch culture". CTYF-Ciencia, Tecnología and Futuro, 2 (2): 7-15.

Boron, D., Deever, W., Atlas, R., McFarland, B., Meyer, J. and Johnson, A., 1999. "Biodesulfurization of gasoline: an assessment of technical and economic feasibility and outline of R and D roadmap toward commercialization". NPRA Annual Meeting, March, San Antonio, Texas, USA .

Cass, B. J., Schade, F., Robinson, C. W., Thompson, J. E. and Legge, R. L., 2000. "Production of tomatoe flavor volatiles from a crude enzyme preparation using a hollow-fiber reactor". Biotechnology and Bioengineering, 67 (3): 372-377. https://doi.org/10.1002/(SICI)1097-0290(20000205)67:3<372::AID-BIT14>3.0.CO;2-N

Denome, S., Oldfield, C., Nash, L. and Young, K. C., 1994. "Characterization of the desulfurization genes from Rhodococcus sp. strain IGTS8". J. Bacteriol., 176: 6707-6716. https://doi.org/10.1128/jb.176.21.6707-6716.1994

Folsom, B. R., Schieche, D. R., Dgrazia, P. M., Werner, J. and Palmer, S. K., 1999. "Microbial desulfurization of alkylated dibenzothiophenes from a hydrodesulfurized middle distillate by rhodococcus erythropolis I- 19". Applied and Environmental Microbiology, 65 (11): 4967-4972. https://doi.org/10.1128/AEM.65.11.4967-4972.1999

Fredrick, C., 2002. "Sulfur reduction: what are the options?". Hydrocarbon Processing, February, 45-50.

Giorno, L. and Drioli, E., 2000. "Biocatalytic membrane reactors: applications and perspectives". Tibtech., 18: 339-349. https://doi.org/10.1016/S0167-7799(00)01472-4

Gray, K., Pogrebinsky, O., Mrachko, G., Xi, L., Monticello, D. and Squires, C., 1996. "Molecular mechanisms of biocatalytic desulfurization of fossil fuels". Nature Biotechnol., 14: 1705-1709. https://doi.org/10.1038/nbt1296-1705

Honda, H., Sugiyama, H., Saito, I. and Kobayashi, T., 1998. "High cell density culture of rhodococcus rhodochrous by pH-Stat feeding and dibenzothiophene degradation". Journal of Fermentation and Bioengineering, 85 (3): 334-338. https://doi.org/10.1016/S0922-338X(97)85685-1

Izumi, Y., Ohshiro, T., Ogino, H., Hine, Y. and Shimao, M., 1994. "Selective desulfurization of dibenzothiophene by Rhodococcus erythropolis D- 1". Appl. Environ. Microbiol., 60: 223-226. https://doi.org/10.1128/aem.60.1.223-226.1994

Janusz, P., 1997. "Solid phase microextraction: theory and practice". Wiley - VCH, Inc., New York, USA .

Kaufman, E., Harkins, J., Rodríguez, M., Tsouris, C. , Severaj and Murphy, S., 1997. "Develoment of an electro-spray biorreactor for crude oil processing". Fuel processing Technology, 52: 127- 144. https://doi.org/10.1016/S0378-3820(97)00022-2

Kilbane, J. J. and Bielaga, B. A., 1990. "Towards sulfur-free fuels". Chem.Tech. Dec., 747-751.

Kirimura, K., Furuya, T., Sato, R., Ishii, Y. Kino, K. and Usami, S., 2002. "Biodesulfurization of naphthothiophene and benzothiophene through selective cleavage of carbon-sulfur bonds by rhodococcus sp. strain WU-K2R". Appl. Environ. Microbiol., 68 (8): 3867-3872. https://doi.org/10.1128/AEM.68.8.3867-3872.2002

Konishi, J., Onaka, T., Ishii, Y. and Suzuki, M., 2000. "Demonstration of the carbon-sulfur bond targeted desulfurization of benzothiophene by thermophilic paenibacillus sp. Strain A11-2 capable of desulfurizing dibenzothiophene". FEMS Microbiology, 187: 151-154. https://doi.org/10.1111/j.1574-6968.2000.tb09152.x

Levy, R., Rappas, A., Decanio, S. and Nero,V., 2001. "UniPures ASR-2 desulfurization process provides cost-effective solution for ultra low sulfur refined products". NPRA Annual Meeting, March.

Madero, A., Mogollón, L., Mora, A. and Osorio, L., 1998. "Selección de microorganismos mesófilos biodesulfurizadores". CTYF-Ciencia, Tecnología and Futuro, 1 (4): 93-100.

Monticello, D. J., 2000. "Biodesulfurization and the upgrading of petroleum distillates". Current Opinion in Biotechnology, 11: 540-546. https://doi.org/10.1016/S0958-1669(00)00154-3

Monticello, D. J., 1998. "Riding the fossil fuel biodesulfirization wave". Chemtech., 28 (7): 38-45.

Ohshiro, T. and Izumi, Y., 1999. "Microbial desulfurization of organic sulfur compounds in petroleum". Biosc. Biotechnol. Biochem., 63 (1): 1-9. https://doi.org/10.1271/bbb.63.1

Piddington, C., Kovacevich, B. and Rambosek, J., 1995. "Sequence and molecular characterization of a DNA region encoding the dibenzothiophene desulfurization operon of rhodococcus sp, strain IGTS8". Appl. Environ. Microbiol., 61: 468-475. https://doi.org/10.1128/aem.61.2.468-475.1995

Puerta, M. A. and Staschenko, H., 2000. "Informe- análisis de los metabolitos producidos durante el proceso de biodesulfurización de la cepa ICP172". Laboratorio de Cromatografía, Universidad Industrial de Santander. Bucaramanaga, Colombia.

Setti, L., Farinelli, P. and Di Martino, S., 1999. "Development in destructive and nondestructive pathways for selective desulfurizations in oil-biorefinig processes". Appl. Microbiol. Biotechnol., 52: 111-117. https://doi.org/10.1007/s002530051496

Vazquez-Duhalt, R., Torres, E., Valderrama, B. and Le Borgne, S., 2002. "Will biochemical catalysis impact the petroleum refining industry?". Energy and Fuels, 16: 1239-1250. https://doi.org/10.1021/ef020038s

Wang, P. and Krawiec, S., 1996. "Kinetic analyses of desulfurization of dibenzothiophene by rhodococcus erythropolis in batch and fed-batch cultures". Applied and Environmental Microbiology, 62 (5): 1670-1675. https://doi.org/10.1128/aem.62.5.1670-1675.1996

Wang, P., Humphrey, A. E. and Krawiec, S., 1996. "Kinetic analyses of desulfurization of dibenzothiophene by rhodococcus erythropolis in continuous cultures". Applied and Environmental Microbiology, 62 (8): 3066-3068. https://doi.org/10.1128/aem.62.8.3066-3068.1996

Yoshitaka, I., Konishi, J., Okada, H., Hirasawa, K., Onaka, T. and Suzuki, M., 2000. "Operon structure and functional analysis of the genes encoding thermophilic desulfurizing enzymes of paenibacillus sp. A11- 2". Biochemical and Biophysical Research Communications, 270: 81-88. https://doi.org/10.1006/bbrc.2000.2370

How to Cite
Acero, J., Berdugo, C., & Mogollón, L. (2003). Biodesulfurization process evaluation with a Gordona rubropertinctus strain. CT&F - Ciencia, Tecnología Y Futuro, 2(4), 43–54. https://doi.org/10.29047/01225383.527

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Published
2003-12-31
Section
Scientific and Technological Research Articles

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