Aqueous - organic phases separation by membrane reactors in biodesulfurization reactions
Abstract
This work presents a membrane bioreactor prototype used to separate emulsion phases formed in the biodesulfurization reaction. Hydrophobic membranes used for the construction of the prototype allow the separation of the organic/watery phases. The separation unit resembles a tube and carcass heat exchanger. By feeding the emulsion through the housing and due to the pressure gradient pushed on the membrane, the organic phase pass through and allow to obtain an organic phase free of cells and water. Several organic phase/watery phase ratios and many cellular concentrations were evaluated. Results indicate that is possible to separate the phases by manipulating the fluid pressure within the bioreactor. This is possible even for cellular concentrations of the order of 7 g/l. The system can also be used as a reaction unit. The biological conversion was evaluated by verifying the presence of 2-HBP, one of the metabolites of the path 4S in the biodesulfurization reaction. This bioreactor configuration has not been explored before for the biodesulfurization process and therefore it represents an innovation in this research area.
References
Belfort, G., 1989. "Membranes and bioreactors: a technical challenge in biotechnology". Biotechnology and Bioengineering, 33: 1047-1066. https://doi.org/10.1002/bit.260330814
Berdugo, C., Mena, J. A., Acero, J. R. and Mogollón, L., 2001. "Increasing the production of desulfurizing biocatalysts by means of Fed-Batch culture". CT&F - Ciencia, Tecnología y Futuro, 2 (2): 7-15.
Bruining, W., 1989. "A general description of flows and pressure in hollow fiber membrane modules", 44 (6): 1441-1447. https://doi.org/10.1016/0009-2509(89)85016-X
Dai, Xiao-Ping, Luo, R. and Sirkar, K., 2000. "Pressure and flux in bead-filled ultrafiltration/microfiltration hollow fiber membrane modules". Biotechnology Progress, 16: 1044-1054. https://doi.org/10.1021/bp000072u
Cass, B. J., Schade, F., Robinson, C. W., Thompson, J. E. and Legge, R. L., 2000. "Production of tomato flavor volatiles from a crude enzyme preparation using a hollow-fiber reactor". Biotechnology and Bioengineering, 67 (3): 372-377. https://doi.org/10.1002/(SICI)1097-0290(20000205)67:3<372::AID-BIT14>3.0.CO;2-N
Deeslie, D. and Cheryan, M., 1981. "A CSTR- Hollow Fiber System for continuous hydrolysis of proteins. Performance and Kinetics". Biotechnology and Bioengineering, 23: 2257-2271. https://doi.org/10.1002/bit.260231009
Giorno, L. and Drioli, E., 2000. "Biocatalytic membrane reactors: applications and perspectives". Tibtech., 18: 339-349. https://doi.org/10.1016/S0167-7799(00)01472-4
Gramer, M., Poeschl, D., Conroy, M. and Hammer, B., 1999. "Effect of harvesting protocol on performance of a hollow-fiber bioreactor". Biotechnology and Bioengineering, November 5, 65 (3): 334-340. https://doi.org/10.1002/(SICI)1097-0290(19991105)65:3<334::AID-BIT11>3.0.CO;2-L
Kaufman, E., Harkins, J., Rodríguez, M., Tsouris, C., Selvaraj, P. and Murphy, S., 1997. "Development of an electro-spray bioreactor for crude oil processing". Fuel Processing Technology, 52: 127-144. https://doi.org/10.1016/S0378-3820(97)00022-2
Kelsey, L., Pillarella, M. and Zydney, A., 1990. "Theoretical analysis of convective flow profiles in a hollow-fiber membrane bioreactor". Chemical Engineering Science, 45 (11): 3211-3220. https://doi.org/10.1016/0009-2509(90)80213-X
Loh, K., Chung, T. and Ang, W., 2000. "Immobilized-cell membrane bioreactor for high-strength phenol waste-water". Journal of Environmental Engineering. January. https://doi.org/10.1061/(ASCE)0733-9372(2000)126:1(75)
Luque, S., Mallubhotla, H., Gehler, G., Kuriyel., Dzenge-leski, S., Pearl, S. and Belfort, 1999. "A new coiled hollow-fiber module design for enhanced microfiltration performance in biotechnology". Biotechnology and Bioengineering, November 5, 65 (3): 247-257. https://doi.org/10.1002/(SICI)1097-0290(19991105)65:3<247::AID-BIT1>3.0.CO;2-H
Ohshiro, T., Hirata, T. and Izumi, Y., 1995. "Microbial desulfurization of dibenzothiopheno in the presence of hydrocarbons". Appl. Microbiol Biotechnol., 44: 249-252. https://doi.org/10.1007/BF00164510
Patankar, D. and Oolman, T., 1990. "Wall-growth hollow-fiber reactor for tissue culture: I. Preliminary experiments". Biotechnology and Bioengineering, 36: 97-103. https://doi.org/10.1002/bit.260360113
Patkar, A., Bowen, B. and Piret, J., 1993. "Protein adsorption in polysulfone hollow fiber bioreactors used for serum-free mammalian cell culture". Biotechnology and Bioengineering, 42: 1099-1106. https://doi.org/10.1002/bit.260420912
Pearson, D., 2002. "Easing wastewater woes through membrane filtration". Chemical Processing, January, pp. 24-27.
Piret, J. and Cooney, C., 1990. "Mammalian cell and protein distributions in ultrafiltration hollow fiber bioreactors". Biotechnology and Bioengineering, 36: 902-910.
https://doi.org/10.1002/bit.260360905
Setti, L., Lanzarini, G. and Pifferi, G., 1996. "Immobilized cells for applications in non-conventional systems". In: Immobilized Cells: Basics an Applications, Wiffels, R., Buitelaar, R., Bucke, C. and Tramper, J., (Eds), 777-784. https://doi.org/10.1016/S0921-0423(96)80106-7
Schröen, C. and Woodley, J., 1997. "Membrane separation for downstream processing of aqueous-organic biocon-versions". Biotechnol. Prog., 13: 276-283. https://doi.org/10.1021/bp970014h
Sehanputri, P. S. and Hill, C. G., 1999. "Biotechnology for the production of nutraceuticals enriched in conjugated linoleic acid: I. uniresponse kinetics of the hydrolysis of corn oil by a pseudomonas sp. lipase immobilized in a hollow fiber reactor". Biotechnology and Bioengineering, September 5, 64 (5).
https://doi.org/10.1002/(SICI)1097-0290(19990905)64:5<568::AID-BIT7>3.0.CO;2-E
Tejada, A., Montesinos, R., and Guzmán, R., 1995. "Bioseparaciones". Sonora, México, Editorial Unison.
Tompkins, K., Murphy, L., Owsenek, B., Pignataro, R. and Rodríguez, A., 2000. "Ultrafiltration membrane polishing system for ship boat treatment of oily wastewater". Naval Sea Systems Command, Crystal City.
Vaidya, A., Halling, P. and Bell, G., 1994. "Surfactant-induced breakthrough effects during the operation of two-phase biocatalytic membrane reactors". Biotechnology and Bioengineering. 44: 765-771. https://doi.org/10.1002/bit.260440613
Downloads
Copyright (c) 2002 Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
