Biotransformation and removal of sulfur from dibenzothiophene using improved biocatalytic methods
Abstract
Three methods for the removal of sulfurfrom dibenzothiophene were evaluated using biocatalytic processes. The methods were a microbial, an enzymatic and a combined one that involves a previous enzymatic oxidation followed by microbial degradation. The byconversion was evaluated over the molecular dibenzothiophene model, obtaining higher byconversion percentages through the combined method. The microorganisms used in this study correspond to several Colombian indigenous strains isolated by direct methods from natural sources, and using a standard strain as positive control. All strains have shown sulfur removal capacity, and organic solvent tolerance. The enzyme used was a hemoprotein with peroxidase activity, Cytochrome C, obtained from equine heart.
References
Crawford D. L. and Gupta R. K., 1990. "Oxidation of dibenzothiphene by Cunninghamella elegans". Curr Microbiol, 21: 229-231. https://doi.org/10.1007/BF02092161
Fedorak, P. M, Semple K. M, Vázquez-Duhalt, R. and Westlake D. W., 1993. Enzyme Microb. Technol., 15 (May): 429 - 37. https://doi.org/10.1016/0141-0229(93)90131-K
Gallagher, J. R, Olson, E. S. and Stanley, D. C, 1993. Microbial desulfiirization of dibenzotiofene: a sulfur-specific pathway, FEMSMicrobiology Letters, 107: 31-36. https://doi.org/10.1111/j.1574-6968.1993.tb05999.x
Hartdegen, E. J., Coburn, J. M. and Roberts, R. L, 1984. "Microbial Desulfurization of Petroleum", CEP.
Izumi, Y, Ohshiro, T., Ogino, H., Hine, Y. and Shimao, M., 1994. "Selective desulfurization of dibenzothiophene by Rhodococcus erythropolis D-1", Appl. Microbiol Biotechnol., 60: 223 -226. https://doi.org/10.1128/aem.60.1.223-226.1994
Kayser, K. J., Bielaga, B. A., Jackowski, K., Odusan, O. and Kilbane II, J.J.1993. "Utilization of organosulfur compounds by axenic and mixed cultures of Rhodococcus rhodochorous IGTS 8", J. Gen Microbiol, 139: 3123-3129. https://doi.org/10.1099/00221287-139-12-3123
Kilbane, J. J. and Bielaga, B.A. 1990. "Toward sulfur-free fuels", Chemtech, 20: 747-51.
Kilbane II J. J. and Woodstock III, 1991. "Mutant Microorganisms useful for cleavage of organic C -S bonds", Patent 5104801.
Kilbane II, J. J and Jackowski, K. 1992. "Biodesulfurization of water-soluble coal -derived material by Rhodococcus rhodochorous IGTS", Biotechnol Bioeng, 40: 1107 -1114. https://doi.org/10.1002/bit.260400915
Kim, H. Y, Kim, T S. and Kim, B. H., 1990. "Degradation of organic sulfur compounds and the reduction of diben-zotiophene to biphenyl and hydrogen sulfide by Desul-fovibrio desulfuricans Mb", Biotechnol lett. 12: 761 -764. https://doi.org/10.1007/BF01024736
Krawiec, S. 1990. "Bacterial desulfurization of thiphones: screening techniques and some speculation regarding the biochemical and genetic", Dev. Ind. Microbiol., 32: 103-114.
Monticello, D. J., 1993. "Biocatalytic desulfurization of petroleum and middle distillates", Environmental Progress, 12 (1): 1-4. https://doi.org/10.1002/ep.670120103
Monticello, D. J., 1994. "Biocatalytic desulfurization", Hydricarbons Processing (February).
Ohshiro, T, Hirata T. and Izumi, Y, 1995. "Microbial desulfurization of dibenzothiophene in the presence of hydrocarbon", Appl. Microbiol Biotechnol., 44: 249 -252. https://doi.org/10.1007/BF00164510
Olson, E. S., Stanley, D. C. and Gallagher, J. R, 1993. "Characterization of intermediates in the microbial desulfurization of dibenzothiophene", Energy & Fuels, 7: 159-164. https://doi.org/10.1021/ef00037a025
Oldfield, C, Pagrebinsky, O.,Simons, J., Olson, E. S. and Kulpa, CH. F 1997. "Elucidation of the metabolic pathway for dibenzothiophene desulfurization by Rhodococcus sp. strainIGTS8(ATCC53968)", Microbiology, 143: 2961-2973. https://doi.org/10.1099/00221287-143-9-2961
Ózbas, T., Durusoy, T, Erincin, E. and Yürüm, Y 1996. "Optimization of the growthparameters of Rhodococcus rhodochorous, a sulfur - removing", Fuel 75 (13): 1556 -1600.
Sorkhoh, N. A., Ghannoum, M. A., Ibrahim. A. S., Stretton, R J. andRadwan, S. S.,1990. "Crude oil and hydrocarbon-degrading strain of Rhodococcus rhodochrous isolated from soil and marine environments in Kuwait", Environment Pollution, 65: 1-17. https://doi.org/10.1016/0269-7491(90)90162-6
Vázquez-Duhalt, R., Westlake, D. W S. and Fedorak P. M., 1993. "Cytochrome C as a bicatalyst for the thiophenes and organosulfides", Enzyme Microb. Technol., 15 (June): 494-499. https://doi.org/10.1016/0141-0229(93)90082-D
Yamada, K, Minoda,Y, Kodama, K, Nakatani, S. and Akasa M, T. 1968. "Microbial conversion of petro-sulfur compounds. Part I. Isolation and identyification of Dibenzothiophene utilizing Bacteria", Agr. Biol . Chem., 32 (7): 840 - 845. https://doi.org/10.1080/00021369.1968.10859154
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