Lactic acid production by Lactobacillus sp. from biodiesel derived raw glycerol

  • Yessica Prada-Palomo Universidad Industrial de Santander, Bucaramanga, Colombia
  • Marly Romero-Vanegas Universidad Industrial de Santander, Bucaramanga, Colombia
  • Pedro Díaz-Ruíz Universidad Industrial de Santander, Bucaramanga, Colombia
  • Daniel Molina-Velasco Universidad Industrial de Santander, Bucaramanga, Colombia
  • Carolina Guzmán-Luna Universidad Industrial de Santander, Bucaramanga, Colombia
Keywords: Lactid acid, Biodiesel, Raw glycerol, Native microorganism, Bioconversion, Fermentation, Nuclear magnetic resonance


Lactic Acid (LA) is a versatile compound with extensive industrial usage. Lactic Acid Bacteria (LAB) are renowned for their capacity to ferment carbohydrates to LA. In the biodiesel industry, bioconversion of residual glycerol to monomers of biopolymers, such as LA, is an alternative for usage of this waste as raw material to produce a high value added biotechnological compound. This research evaluates LA production through fermentation processes using a Lactobacillus sp. native strain and raw glycerol from the biodiesel industry. Native strain was isolated from the processing of dairy, fruit, and vegetable products and through cultures in Man, Rogosa & Sharpe agar (MRS). After an adaptation phase, the fermentative capability of the strain was evaluated through chemical quantification of metabolites using Nuclear Magnetic Resonance (1H-NMR); fermentative potential was compared with a Lactobacillus sp. reference strain (ATCC 7469). The native strain Lactobacillus sp. CYP4 showed a higher bioconversion potential (LA concentration: 39.41 mM, conversion percentage: 39.27%, at 24 h, volumetric productivity: 1.28 g.L-1.h-1, yield: 0.71 g·g-1) from raw glycerol, when compared to the reference strain.


Álvarez, M., Medina, R., Pasteris, S., Strasser de Saad, A. & Sesma, F. (2004). Glycerol metabolism of Lactobacillus rhamnosus ATCC 7469: Cloning and expression of two glycerol kinase genes. J. Mol. Microbiol. Biotechnol., 7(4), 170-181.

Claisse, O. & Lonvaud-Funel, A. (2000). Assimilation of glycerol by a strain of Lactobacillus collinoides isolated from cider. Food Microbiol., 17(5), 513-519.

Da Silva, G. P., Mack, M. & Contiero, J. (2009). Glycerol:A promising and abundant carbon source for industrial microbiology. Biotechnol. Advances, 27(1), 30- 39.

Du, W., Li, W., Sun, T., Chen, X. & Liu, D. (2008). Perspectives for biotechnological production of biodiesel and impacts. Appl. Microbiol. Biotechnol., 79(3), 331-337.

El-Ziney, M. G., Arneborg, N., Uyttendaele, M., Debevere, J. & Jakobsen, M. (1998). Characterization of growth and metabolite production of L. reuteri during glucose/ glycerol cofermentation in batch and continuous cultures. Biotechnol. Lett., 20(10), 913-916.

European Biodiesel Board. (2011). 2010-2011: EU biodiesel industry production forecasts show first decrease in 2011 since data is gathered. [Press release web]. (October 18th 2011). Available at:

Federación Nacional de biocombustibles de Colombia. Sector de los Biocombustibles. [Accessed: Nov. 01, 2012]. Available at: .

Felis, G. E., Dellaglio, F. & Torriani, S. (2009). Chapter 15: Taxonomy of probiotic microorganisms. In: Charalampopoulos. D. & Rastall. R. A. Prebiotics and probiotics science and technology. New York: Springer Sience + Business media. 591-637.

Fischer, G., Hizsnyik, E., Prieler, S., Shah, M. & Van Velthuizen, H. (2009). Biofuels and Food Security. International Institute for applied systems analysis (IIASA). The OPEC Fund for International Development (OFID). Vienna: Stiepan Druck GmbH.

Garai-Ibabe, G., Ibarburu, I., Berregi, I., Claisse, O., Lonvaud- Funnel, A., Irastorza, A. & Dueñas, M. T. (2008).

Glycerol metabolism and bitterness producing lactic acid bacteria in cidermaking. Internat. J. Food Microbiol.,
121(3), 253-261.

Hofvendahl, K. & Hahn-Hägerdal, B. (2000). Factors affecting the fermentative lactic acid production from
renewable resources. Enzyme Microb. Technol., 26(2-4),87-107.

Johnson, D. T. & Taconi, K. A. (2007). The glycerin glut: Options for the value-added conversion of crude glycerol resulting from biodiesel production. Environ. Progress.26(4), 338-348.

König, H. & Berkelmann-Löhnertz, B. (2009). Chapter 25: Maintenance of wine-associated microorganisms. In: Biology of Microorganisms on Grapes. in Must and in Wine. Germany: Springer-Verlag Berlin Heidelberg. 451-468.

Lüthi-Peng, Q., Dileme, F. B. & Puhan, Z. (2002). Effect of glucose on glycerol bioconversion by Lactobacillus
reuteri. Appl. Microbiol. Biotechnol., 59(2-3), 289-296.

National Biodiesel Board. EPA Release June-Biodiesel Volume. [Press release web]. (31 July 2012). [Accesed:
Nov. 11, 2012]. Available at: epa-releases-junebiodiesel-volume>

Okano, K., Tanaka, T., Ogino, C., Fukuda, H. & Kondo,A. (2010). Biotechnological production of enantiomeric
pure lactic acid from renewable resources: recent achievements. perspectives. and limits. Appl. Microbiol.
Biotechnol., 85(3), 413- 423.

Sauvageot, N., Gouffi, K., Laplace, J. M. & Auffray, Y. (2000). Glycerol metabolism in Lactobacillus collinoides: production of 3-hydroxypropionaldehyde. A precursor of acrolein. Int. J. Food Microbiol., 55(1-3), 167–170.

Serna-Cock, L. & Rodríguez-de Stouvenel, A. (2005). Producción biotecnológica de ácido láctico: estado del arte. Ciencia y tecnología alimentaria, 5(001), 54 – 56.

Sims. B. Biodiesel: a global perspective. Biodiesel Magazine. [Press release web. 2007]. < http://www.biodieselmagazine. com/article.jsp?article id=1961>

Wee, Y. J., Kim, J. N. & Ryu, H. W. (2006). Biotechnological production of Lactid Acid and its recent applications. Food Technol. Biotechnol., 44(2), 163-172.

Yazdani, S. S. & Gonzalez, R. (2007). Anaerobic fermentation of glycerol: a path to economic viability for the biofuels industry. Current Opin. Biotechnol., 18(3), 213- 219.
How to Cite
Prada-Palomo, Y., Romero-Vanegas, M. ., Díaz-Ruíz, P. ., Molina-Velasco, D. ., & Guzmán-Luna, C. . (2012). Lactic acid production by Lactobacillus sp. from biodiesel derived raw glycerol. CT&F - Ciencia, Tecnología Y Futuro, 5(1), 57–66.


Download data is not yet available.
Scientific and Technological Research Articles


QR Code

Some similar items: